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The numbers of assembled unigenes/transcripts that were expressed (cut-off value at FPKM ≥ 0.05) in male and female P. viridis were similar, however strong variations were observed for unigene/transcript abundance among tissues.
Hughes and co-workers reporting in Cell [8] verified the validity and significance of genes expressed at cut-off levels of 1.2 and above in expression studies that they carried out on E. coli.
Our rational for establishing a cut off value is that any patient who expresses above the cut off level could be selected as a candidate for anti-estrogen therapies and could be considered to have good prognosis.
FP = false positives: genes expressed above cut-off in either RNAseq or 3Pseq and resulting in a 'no signal' FISH annotation (see Figure 1 figure supplement 2A)).
FN = false negatives: genes expressed below cut-off in either RNAseq or 3Pseq and giving a 'ubiquitous' or 'specific' in FISH signal.
TP = true positives: genes expressed above cut-off in either RNAseq or 3Pseq and giving a 'ubiquitous' or 'specific' in FISH signal.
One possibility is that probes were specific to different isoforms of the gene); pn-status: comparison of sequencing and FISH results (TN = true negative: genes expressed below cut-off in either RNAseq or 3Pseq and giving a 'no signal' in FISH experiments.
The UN humanitarian affairs chief has expressed deep concern for communities cut off in Syria by months of fighting between government and rebel forces.
In some cases, the interviewer cut off replies expressed in the first person and immediately returned the conversation to Flora's perspective.
In order to test the presence of expressed repetitive sequences, BLASTN (e-value cut off ≤ 10e-50) searches were performed against all Viridiplantae Repbase (reference database of eukaryotic repetitive DNA).
"Low" expression was assigned as significantly expressed (i.e., > 0.125 RPKM cut off, but less than 1 RPKM, corresponding to < 1 mRNA/cell [ 23]).
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