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All cells in the region of the TC domain in the DT expressed Cut and Vein.
These cells also ectopically expressed Cut, an additional marker of myoblasts, further confirming the acquisition of mesoderm identity and the occurrence of EMT.
Cells on the trunk side of this border expressed Ser and Delta; cells on the other side expressed Cut and Vein.
All of the NRE-lacZ expressing dorsal branch cells expressed Kni; all of the NRE- lacZ expressing TC cells expressed Cut.
Similar(56)
The numbers of assembled unigenes/transcripts that were expressed (cut-off value at FPKM ≥ 0.05) in male and female P. viridis were similar, however strong variations were observed for unigene/transcript abundance among tissues.
(J, K ) Clones that ectopically expressed Sal in the TC ectopically expressed Delta, which is not normally expressed in the TC (J, J' ), but did not express Cut, which is normally expressed in the TC (K, K' ).
Thus, in contrast to clones of N intra-expressing cells that up-regulate Wg and Cut in all wing blade regions, clones of UAS- dpn RNAi; E spl Xp UAS -N intra located in the proximal regions of the wing pouch either did not express Cut and Wg or did so at very low levels (Table 1).
Because ecdysone activation induces dendrite pruning first before regrowth (Kuo et al., 2005), and overexpressed Cp1 protein in larval ddaC neurons remained cytoplasmic and gave no obvious phenotype, we tested this possibility of context specificity by expressing Cut during dendrite development.
Recent genetic fate-mapping experiments have suggested that, on the population level, progenitors expressing cut-like homeobox 2 (CUX2), another marker specific for callosal and other superficial-layer neurons, exclusively produce superficial-layer neurons (Franco et al., 2012), although conflicting results were subsequently reported (Guo et al., 2013).
Cut-HA expressed in cut-mutant ddaC neurons also resulted in greatly reduced higher-order dendrites in third-instar larvae.
In addition, sap continued to be spontaneously expressed from cut stems of excised roots several hours after mercury application demonstrating the generation of strong root pressure in the treated roots.
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