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Indirect immunofluorescence assays in transfected HeLa cells indicated that the recombinant DNAs (rDNAs) could be expressed correctly and had antigenicity.
It is shown that the photocathode life time cannot be expressed correctly in the units of the collected anode charge.
After verifying that all mutant PB2 proteins were expressed correctly (Fig. S1), their nuclear import in HeLa cells was tested by indirect immunofluorescence using PB2-specific antibodies.
All participants receive a verbatim transcript and are asked to verify whether their opinion is expressed correctly (member check).
These results show that αReps are expressed, correctly folded and bind specifically their target in eukaryotic cells.
First, the decreased amplitude could reflect a decrease in the number of channels that are expressed correctly in the membrane as compared to wild type.
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As these quantifications ignore the size of the transduced area which directly affects the absolute number of positive cells per section, we quantified the density of cones expressing correctly the GNAT2 marker in the region of wt RPE65 expression.
The results indicate that FPV recombinants can express correctly the E6 and E7 oncoproteins, and they should represent appropriate vectors for the expression of these oncoproteins in human cells.
One bottleneck for studying membrane proteins is the difficulty in expressing correctly folded and stable proteins, which often requires extensive protein engineering and multiple rounds of optimization, a time and resource intensive process.
Attempts to express correctly folded and localised BesB using N- and C-terminal his- and strep-tags as well as fusions with maltose-binding protein and GFP were repeatedly unsuccessful.
Visual perception is used to tell in particular cases whether the rule thus expressed is correctly implemented, in the context of prior knowledge that the rule is correct.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com