Interestingly, despite achieving high expression levels, the exclusively nuclear-localised BAG-1L isoform was only able to significantly inhibit radiation-induced apoptosis in the Dox-treated Saos-2/Rb/DC/R5 cells when Rb is expressed, consistent with a requirement for Rb expression for the anti-apoptotic function of BAG-1L.
Interestingly, a sole oligosaccharide ABC transporter-specific ATP-binding protein was identified in the genome and found to be constitutively highly expressed, consistent with a single ATP-binding protein energizing multiple oligosaccharide ABC transporters as previously described [ 56].
As the two 6S RNAs, tmRNA was highly expressed consistent with its role in recovery from a variety of stresses and interestingly both, 6S2 RNA and tmRNA, showed evidence of antisense transcription (Table S8).
Arginine butyrate-treated mice gene expression profiling experiments revealed that several genes that control cell proliferation, growth and differentiation are differentially expressed consistent with its histone deacetylase inhibitory activity when compared to control (saline-treated) mdx mice.
Whereas BCL2 and MCL1 were differentially expressed, consistent with our previous report [ 46], all 3 cell lines expressed BCL2A1.
Furthermore, genes involved with transcriptional regulation and DNA/RNA binding are also differentially expressed consistent with potential gene silencing processes.
The results demonstrated that all the 5 selected genes were clearly expressed in trichomes, 3 were highly expressed and 2 were weakly expressed, consistent with the microarray data.
However, after 24 h, when FA concentration was high, the DGA1 gene was strongly expressed, consistent with the major contribution of its product to TAG formation.
However, after 24 h, when FA concentration was high, the DGA1 gene was strongly expressed, consistent, again, with its major contribution to TAG formation.
Separating genes into quartiles based on RNA Polymerase II occupancy in their coding regions (Kim et al. 2010) revealed that highly expressed genes exhibit a greater increase in nucleosome spacing in the presence of K. lactis Chd1 than the lowest expressed genes, consistent with a potentially cotranscriptional role for Chd1 in nucleosome spacing.
In contrast, basal subtype cancers rarely expressed TOX3, consistent with a previous report [ 19].
