We found that AID interacts with the calcium and integrin binding protein 1 (CIB1), a 22 kDa regulatory protein that is broadly expressed, comprised of four EF hand motifs (two of which bind calcium), and required for spermatogenesis in mice [22], [23].
The fusion protein expressed comprises CD44s with luciferase at its C-terminal end.
The two most common variants of the polymorphism are typically defined as a "short-allele" (i.e., S-allele, low expressing) comprising 14 copies and a "long-allele" (i.e., L-allele, high expressing) comprising 16 copies.
Comparison of the gene expression profiles of CF4ab to CF4ac revealed 77 differentially expressed transcripts, comprising 29 unique genes, with criteria of P < 0.05, |FC| > 1.5 and FDR < 0.600 (Table 1).
The least expressed gene comprising the chimera was chosen to evaluate the expression, as the limiting factor for its formation.
The initial filtering criteria of p < 0.05 for sex or age difference with an absolute fold-change greater than or equal to 1.5 in relative expression at any age was used to define a set of 7,951 differentially expressed features comprising 3,770 unique Entrez Gene IDs or differentially expressed genes (DEGs).
Venom gland transcriptomic analysis of an adult individual identified toxins belonging to 18 protein families, with three-finger toxin isoforms being the most abundantly expressed transcripts, comprising 94% of all venom protein transcript reads.
Differentially expressed genes comprising enrichment clusters based on biological function between the WT and R57. Figure S4.
Maternally expressed genes, comprising 35% of all genes, are largely housekeeping genes which encode proteins required for essential cellular functions [ 38].
Differentially expressed genes comprising signature 1. Genes within signature 1 and sub-signatures defined at more stringent branch thresholds are ranked based on overexpression in localised prostate cancer within dataset GSE3325.
