We found that AID interacts with the calcium and integrin binding protein 1 (CIB1), a 22 kDa regulatory protein that is broadly expressed, comprised of four EF hand motifs (two of which bind calcium), and required for spermatogenesis in mice [22], [23].
As senescence progresses from partial to fully open flower, 58 TFs are differentially expressed comprising the "WRKY" family with 6 unigenes, followed by the "MYB" and the "AP2/EREBP" family with 4, the "bHLH" TFs with 3, and the "auxin response", "GATA" and "kinesine" with 2 unigenes.
The two most common variants of the polymorphism are typically defined as a "short-allele" (i.e., S-allele, low expressing) comprising 14 copies and a "long-allele" (i.e., L-allele, high expressing) comprising 16 copies.
The expressed protein comprises 1204 amino acids with a calculated molecular mass of 131 kDa (unglycosylated) and a pI of 6.03.
The final list of differentially expressed genes comprises 674 transcripts (Additional file 1).
Assuming that the expressed proteome comprises approximately 10,000 expressed proteins [ 7], we can make a rough estimate of proteome coverage from small sample runs.
The final category of highly expressed genes comprises those involved in immune function, e.g. thymosin β [ 68], α macroglobulin and a mannan-binding lectin associated serine protease, which is a complement control module.
Comparison of the gene expression profiles of CF4ab to CF4ac revealed 77 differentially expressed transcripts, comprising 29 unique genes, with criteria of P < 0.05, |FC| > 1.5 and FDR < 0.600 (Table 1).
The least expressed gene comprising the chimera was chosen to evaluate the expression, as the limiting factor for its formation.
The initial filtering criteria of p < 0.05 for sex or age difference with an absolute fold-change greater than or equal to 1.5 in relative expression at any age was used to define a set of 7,951 differentially expressed features comprising 3,770 unique Entrez Gene IDs or differentially expressed genes (DEGs).
