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We found that AID interacts with the calcium and integrin binding protein 1 (CIB1), a 22 kDa regulatory protein that is broadly expressed, comprised of four EF hand motifs (two of which bind calcium), and required for spermatogenesis in mice [22], [23].
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The fusion protein expressed comprises CD44s with luciferase at its C-terminal end.
As senescence progresses from partial to fully open flower, 58 TFs are differentially expressed comprising the "WRKY" family with 6 unigenes, followed by the "MYB" and the "AP2/EREBP" family with 4, the "bHLH" TFs with 3, and the "auxin response", "GATA" and "kinesine" with 2 unigenes.
The two most common variants of the polymorphism are typically defined as a "short-allele" (i.e., S-allele, low expressing) comprising 14 copies and a "long-allele" (i.e., L-allele, high expressing) comprising 16 copies.
TGM-2, a family member of transglutaminases, is a ubiquitously expressed protein comprised of a single ∼76 kDa polypeptide.
The most highly expressed genes comprised almost exclusively genes involved in proteolysis and peak transcript accumulation occurred between 8 and 16 DAP.
A subset of the entire set of 1081 differentially expressed genes, comprised of growth factors, hormones and their receptors was evaluated for their ability to form a sub-network of regulatory connections.
Comparison of the gene expression profiles of CF4ab to CF4ac revealed 77 differentially expressed transcripts, comprising 29 unique genes, with criteria of P < 0.05, |FC| > 1.5 and FDR < 0.600 (Table 1).
After 10 days in culture, the number of cells expressing Fgfr1 and -2 was significantly decreased to 15% of the total cell number whereas those expressing Fgfr3 comprised a significant portion of the population suggesting that Fgfr3 is increased during the process of cellular differentiation [22].
The least expressed gene comprising the chimera was chosen to evaluate the expression, as the limiting factor for its formation.
The initial filtering criteria of p < 0.05 for sex or age difference with an absolute fold-change greater than or equal to 1.5 in relative expression at any age was used to define a set of 7,951 differentially expressed features comprising 3,770 unique Entrez Gene IDs or differentially expressed genes (DEGs).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com