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NT2 cells expressed ChAT which was significantly increased (12-fold) in NT2N (Fig. 4B).
As a caveat to our premotor tracing strategy, if any premotor neurons expressed ChAT, rabies virus could be complemented again, resulting in spurious two-step labeling.
Medial to the striatum, a distinctive group of GFP+ cell is ChAT−; these cells presumably expressed Chat (and, therefore, Cre ) in the early postnatal period and then repress Chat expression in adulthood.
Similar(57)
Following NGF treatment, the proportion of neurons expressing ChAT was increased (25.0±4.7%) compared with untreated cells (17.5±6.4%) (Figure 1C).
We determined whether the SCCs express ChAT and VAChT, two critical elements for synthesis and packaging of ACh, a potential transmitter.
Seven days of NGF induction resulted in efficient generation of cells expressing ChAT measured by ICC.
Preterm placentae strongly express ChAT in the cytotrophoblast as well as some stromal elements.
These cells have not been identified but may represent placental macrophages (Hofbaur cells) since they are known to express ChAT.
For each field of view, the ratio of ChAT-positive cells to total cells was calculated as the frequency of cells expressing ChAT.
Curiously, although the Midgut Junction DH31 expressing cells express the ChaGal4 reporter (Fig 4B), they do not express CHAT protein (Fig 4A, C).
The ability of multiple placental cell types to express ChAT, as indicated by this study, suggests it potentially regulates a variety of cell functions within the placenta.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com