An empirical Bayes analysis of variance approach [ 32] was then used on the normalised data to separate genes that were differentially expressed between samples from genes with equal expression level across all samples.
The first steps in the statistical analysis of gene expression data are data normalization and determination of the genes that are differentially expressed between samples.
Finally, even when dozens of barcoded individual cDNA libraries are sequenced in a single lane, the vast majority of transcripts in each sample can be detected and numerous genes differentially expressed between samples can be identified.
Genes differently expressed between samples from African Americans and Caucasians regardless of age were enriched for myeloid genes, while the transcripts that differed in expression between younger African Americans and younger Caucasians were enriched for those specific for B-cells.
We therefore hypothesize that a sizeable proportion of the mRNA differentially expressed between samples from patients during a relapse and during remission could be regulated by miRNAs.
To assess whether this strategy was effective we have performed unsupervised hierarchical clustering of the miRNAs that were differentially expressed between samples (21 miRNAs).
