We thus ensured that the Ade2 protein was expressed at the lower limit and indirectly estimated the lower limit from the TEV plasmid copy number.
We thus believe that TIPI-gTOW is a method by which a cellular condition can be constructed with the target protein expressed at the lower limit still supporting cellular function.
Using TIPI-gTOW, we successfully constructed a strain in which GFP-TDegFAde2 was expressed at the lower limit, just sufficient to support cellular growth under the -Ade condition by accelerating degradation by TEV protease.
A positive correlation between transcriptional compensation of a deletion and overall expression level (fig. 4 A ) can be explained by the existence of genes, which are constantly expressed at the highest possible level, limited by gene-unspecific factor such as the availability of RNA polymerase complexes or individual transcription factors.
The general amino acid permease Gap1 is degraded in the vacuole under rich nutrient conditions but is expressed at the plasma membrane under nutrient limiting conditions such as in the presence of proline as the sole nitrogen source (De Craene et al., 2001) (Fig. 3A).
Lipolysis pathways are expressed at the beginning of the ripening period; gene expression is limited at day 5 but increased at days 9 and 15.
We find that robust mCrem expression correlates with a high level of tamoxifen-independent Cre activity, while clones expressing mCrem at the limit of western blot detection exhibit extremely tight regulation.
In this report, we show that robust mCrem expression correlates with a high level of tamoxifen/OHT-independent Cre activity, while clones expressing mCrem at the limit of western blot detection exhibit extremely tight regulation.
In the rebel-held city of Misrata in western Libya and on the eastern front with the rebels around the oil town of Brega, Qaddafi forces continued to hammer rebels with rockets, artillery and mortars, as rebel leaders expressed exasperation at the limits of NATO's support.
Further evidence is required to support these assertions since, although most cofactors are expressed at limiting levels, they have been demonstrated to be widely expressed throughout cell types.
