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One solution is to study the gene expressed at a single ectopic site in cis.
Such a composite end point needs to be expressed at a single concordant age.
Mauzy et al. (2012) noted subsets of genes that are expressed at a single time point only.
Among the former 4129 genes, 3240 genes were preferentially expressed at a single developmental stage, 783 were expressed preferentially at two developmental stages, and 106 at more than two stages.
However, most of these genes were not selected by EPIG as belonging to any of the identified patterns, possibly because the selection of these genes is differentially expressed at a single time point in the previous analysis.
Genes that were identified as being expressed at a single stage of the asexual cell cycle (either ring, trophozoite, or schizont stage) and that were expressed at relatively low levels at the gametocyte stage (bottom 70% expression levels of all genes) were included in the stage-specific expression groups.
Similar(54)
DM2 is expressed as a single transcript in adult flies.
An analysis of DE transcripts of each time point after conditioning showed that the majority of the DE transcripts, i.e. 1759 transcripts of N. vitripennis (71.6%) and 1678 transcripts of N. giraulti (75.6%), were differentially expressed at only a single time point, which indicates substantial temporal differences in gene expression patterns after conditioning for both species.
That is, rather than making a library of the entire transcriptome, we made libraries from transcripts from a single gene expressed at a low level.
Housekeeping genes (HKG) are constitutively expressed in all tissues while tissue-enriched genes (TEG) are expressed at a much higher level in a single tissue type than in others.
In the cortex vs. the other two regions, two genes (5%) were expressed at a higher level, and no single gene was highly expressed in the hippocampus.
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