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Because both alleles are expressed at a relatively equal magnitude, the expression is biallelic.
The results of temporal expression profile analysis indicated that PfFAD2 is expressed at a relatively constant level throughout most stages of seed development up to 42 DAP, but drops about 95% by the latest stage sampled, 49 DAP.
In agreement with the fact that ADAR3 is brain specific in the adult and that ADAR1 p110 mRNA isoform is expressed at a relatively high level in the brain [7], [42], their expression levels in the neuronal culture were higher compared to EBs culture (2.17 and 1.55 folds, respectively; Figure 3A).
Interestingly, it was noted that all four HD-tuins family members expressed at a relatively similar level in somatic embryos and shared a similar expression pattern during in vitro embryo development.
In addition, tissue distribution revealed that miR-181 is expressed at a relatively low level.
In contrast, BtDef was highly expressed in midgut and salivary, and expressed at a relatively lower level in the ovary and fat body in Bemisia tabaci (Wang et al. 2013b).
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In order to examine this possibility in our dataset, we tested whether those genes express at a relatively lower level by exploring current human EST datasets.
Detection of genes which are known to express at a relatively low level in fly brain, diss (CG4211), dnc (CG32498), dco (CG2048), per (CG2647), for example [ 46], as differentially expressed genes in PTZ time series demonstrated that our expression profiling was efficient.
Dhh and Ihh were expressed at relatively higher levels than Shh at E8.5 and E9.5, but Shh was expressed at a higher level than either Dhh or Ihh at E11.5; Ptc1, Gli2, and Gli3 were expressed at relatively higher levels than Smo (Supplementary Figures 1e and f).
Furthermore, Kubis et al. (2004) showed that atTOC159 was strongly expressed in 10-day-old leaves, atTOC90 was expressed at a uniformly moderate level in leaves/inflorescence/roots, whereas atTOC132 and atTOC120 were expressed at a uniformly low level but relatively prominent in inflorescence and roots.
We also identified putative housekeeping genes (HKGs) that showed little variation in expression but were expressed at relatively high levels.
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