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In the cells in which recombination produced an in-frame TCRα, the protein was expected to be first expressed at a point in development similar to endogenous TCRα chains.
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Two factors account for the lack of sensitivity of serological tests at the single-animal level, i) titres of specific antibodies at an early stage of infection and in chronic carriers can be very low, and ii) high variability in antigen expression by MmmSC in vivo, where not all relevant proteins are expressed at a given point in time [ 24, 34].
We find that Sonic Hedgehog (Shh) is expressed at a choice point in the course of the rostral dopaminergic projections.
To investigate potentially co-regulated genes on a more targeted scale (i.e. not necessarily computationally clustered), we examined several groups of functionally-related genes or genes expressed at a specific point in time, starting with ribosomal proteins.
In systems developmental biology where characterization of complexity of various time course data likely leads to address inference of temporal dynamic patterns from transcriptome, we are not often really interested in exactly how only a single gene is temporally differentially expressed at a particular time point or period.
Gene lists were not overlapping, meaning that no gene was differently expressed at a significant level at several time points.
In zebrafish, most highly tissue-specific miRNAs are expressed during embryonic development; approximately 30% of all miRNAs are expressed at a given time point in a given tissue [13].
Since UL83-EYFP and UL32-EYFP are expressed at a later time point than IE2-EYFP it is plausible that these substances act during the early or late phase.
Genes that are differentially expressed at a specific time point may be involved in lineage commitment.
Mauzy et al. (2012) noted subsets of genes that are expressed at a single time point only.
However, most of these genes were not selected by EPIG as belonging to any of the identified patterns, possibly because the selection of these genes is differentially expressed at a single time point in the previous analysis.
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