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Expression levels were normalized to hypoxanthine phosphoribosyltransferase 1 (HPRT1) expression as it was consistently expressed at a moderate level in our arrays and has previously been used as a canine housekeeping gene [ 24] (primer sequences courtesy of Dr. Luke Wittenburg, CSU).
In contrast to the expression pattern or AtNPR3 in Arabidopsis, TcNPR3 only expressed at a moderate level in developing flowers, possibly because cacao is self-incompatible plant, so the tissue specificity might be different in this tropical tree.
The dbpBA operon is expressed at a moderate level during mammalian infection [17], [19] so their expression can be dramatically increased via genetic manipulation.
Thus, our calculation that KRT1 mRNA is present at ∼30 60 copies per cell is consistent with it being expressed at a moderate level in the confluent primary HEK cells.
FSTL3 is also expressed at a moderate level by the pancreas and other adult tissues.
TRPM7 is expressed at a moderate to high level in the majority of pancreatic adenocarcinoma cases (Fig. 2A,B).
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Furthermore, Kubis et al. (2004) showed that atTOC159 was strongly expressed in 10-day-old leaves, atTOC90 was expressed at a uniformly moderate level in leaves/inflorescence/roots, whereas atTOC132 and atTOC120 were expressed at a uniformly low level but relatively prominent in inflorescence and roots.
Although the most highly expressed genes tend to have a lower H3K9me2 level than other genes, this trend does not extend to genes expressed at a low-moderate level.
LTP3 is expressed at a low to moderate level and exhibited a similar expression pattern to LTP2.
The NGF1C transcription factor (also known as EGR4) is expressed at a low to moderate level in the adult cortex and has been shown to be genetically linked to schizophrenia (37) and up-regulated by the antipsychotic, aripiprazole (38).
Among the 60 expressed NTs with ORFs, 55% were expressed at a low level in all 18 tissues, and 45% were expressed at moderate or high levels in one or more tissues (Table 5).
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