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Luciferase activity was measured in the presence of luciferin and ATP according to the manufacturer's instructions using a Lumat LB 9501 luminometer (Berthold, Nashua,N.H ., and was expressed as Reference Luciferase Units (RLU) per milligram of protein.
Moreover, hAAT expressed as reference protein in human amniocyte cell lines is fully glycosylated and sialylated.
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Data were normalized to the endogenous reference HPRT (hypoxanthine phosphoribosyl transferase); then, using the Ct (comparative cycle threshold) method, the amount of RNA transcripts were expressed as a reference to a standardized unstimulated microglia sample.
Relative quantification was accomplished by normalizing raw CT values to the reference gene expressed as target/ reference ratios [ratio = E target(C T target)/E reference(C T reference)] where E represents the empirically determined efficiency estimated for each reaction using LinRegPCR software [ 18].
Normalisation of gene expression was performed using GAPDH as reference gene and data were expressed as ratio to reference samples.
Finally, the longitudinal strain is expressed as follows with reference to Fig. 2.
All values were expressed as percentage of reference values [ 23].
In order to assess the pulmonary function, we measured static lung volumes which were expressed as percentage of reference values [ 23].
First, have a clear rationale for each advisory committee expressed as terms of reference, and consider the best balance between committees and individual consultation with experts.
In addition, sum of four skin-folds were expressed as percentage of reference values of Dutch sedentary males and females of similar ages (Vos 2001).
The dietary intakes of all nutrients were expressed as mg/day per "reference man" when comparing to the results of non pregnant subjects in the 2002 NNHS.
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