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The double fusion reporter gene can be stably expressed and does not alter hESC and hESC-CM characteristics (Figure 5a and 5b, Figure S3).
However, Tbx1 is not expressed and does not function in NC cells.
GOLGA3 is a golgi apparatus gene which is ubiquitously expressed and does not appear to have any specificity that would make it likely to contribute to SSc pathogenesis based on current knowledge [ 47].
Thus although Rac3 is expressed and does activate ERK in MCF-7, this does not result in increased NF-κB activation, because of the very small amounts of the two active subunits of NF-κB expressed in these cells.
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However, highly expressed genes tend to be constitutively expressed and do not overlap significantly with the group of genes that are transiently regulated during stress adaptation.
Orthologs for Arabidopsis genes involved in trichome development were only very weakly expressed and did not vary considerabley between the two genotypes.
While FUS3 transcript levels and, possibly, protein activity remained stable in developing soybean embryos, many SV pairs of ABA-related and FUS3-regulated genes were differentially expressed and did not co-express with FUS3.
Again, the W293 mutant was difficult to express, and did not cause relocalization to theER/perinuclear region (results not shown).
The optimization criteria were level of expression (does it cause inversion when expressed) and leakiness (does it cause inversion when not expressed).
Figure 2c demonstrates that it successfully detects differentially expressed regions and does not detect equally expressed regions between 2a and b.
Varying views were expressed and I did vote for action on Isis and that followed a resolution that said that.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com