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Despite the fact that these progerin-sensitive genes remain lowly expressed and do not change expression dramatically upon the presence of progerin, additional steps might be required for gene activation in disease situations.
In addition, our results indicate that secretion of alkaline phosphatase was increased to a similar extent above control after expression of GFP TRPC3 and FLAG-tagged TRPC7, which are constitutively expressed and do not require the addition of heavy metals.
However, highly expressed genes tend to be constitutively expressed and do not overlap significantly with the group of genes that are transiently regulated during stress adaptation.
As demonstrated by Zhang et al. (2011) [ 35] there are some miRNA* that are low expressed and do not necessarily appear in sequenced libraries, but the complementary mature miRNA sequences were in fact miRNA.
Genes of organisms in nearly constant conditions are deleted, or are constitutively expressed and do not require transcription control, as was experimentally demonstrated for Buchnera [ 39] and Rickettsia [ 40].
The development of vectors for conferring a genetically encoded bioluminescent/fluorescent phenotype onto microorganisms allows the monitoring of any population provided the reporter genes are sufficiently and stably expressed and do not alter the ecological fitness and competitiveness of the host species.
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The double fusion reporter gene can be stably expressed and does not alter hESC and hESC-CM characteristics (Figure 5a and 5b, Figure S3).
However, Tbx1 is not expressed and does not function in NC cells.
Orthologs for Arabidopsis genes involved in trichome development were only very weakly expressed and did not vary considerabley between the two genotypes.
While FUS3 transcript levels and, possibly, protein activity remained stable in developing soybean embryos, many SV pairs of ABA-related and FUS3-regulated genes were differentially expressed and did not co-express with FUS3.
Thus although Rac3 is expressed and does activate ERK in MCF-7, this does not result in increased NF-κB activation, because of the very small amounts of the two active subunits of NF-κB expressed in these cells.
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