All tested molecules showed a concentration-dependent enhancement in fibronectin-mediated adhesion of K562 and SK-MEL-24 cells; in particular 1, expressed a higher affinity towards α5β1 integrin (EC50 of 12 nM) and 2 was more selective for integrin αvβ3 (EC50 of 11 nM).
These data suggest that CD4+ and CD8+ T cells with memory phenotypes express a high affinity ligand for CD62P and potentially can bind to CD62P expressing platelets.
For the functional assay, we used a rat basophil leukemic cell line (RBL SX-38) that expresses a high-affinity human IgE receptor (FcεR1) (Dibbern et al. 2003).
In contrast with the situation found in human platelets, murine platelets lack PAR1 and instead express a high-affinity thrombin-binding receptor, PAR3, in addition to PAR4 which binds thrombin with lower affinity [ 153].
The H-RS cells originate from transformed pre-apoptotic germinal centre B-cells that have lost their capacity to express a high-affinity B-cell receptor (Kuppers et al, 1994; Brauninger et al, 2006).
On their surface Tregs constitutively express CD25, a high affinity receptor for the cytokine interleukin-2 (IL-2).
A lower affinity of the CEH-14 homodimer for the mbr-1 promoter may avoid mbr-1 being expressed wherever ceh-14 is expressed while a higher affinity of the CEH-14/CEH-63 CEH-14/CEH-63 CEH-14/CEH-63nscriptional activation specifically in DVC.
NAT2 codes for a protein predominantly expressed in liver with a high affinity for sulfamethazine and other polymorphically metabolized drugs.
SHM is required as a basis for the selection and proliferation of B cells expressing a BCR with a high affinity for antigen, following interaction with follicular dendritic cells [ 8, 9].
The macrophage mannose receptor (MMR, CD206), a C-type lectin receptor, is ubiquitously expressed on macrophages and has a high affinity for mannose oligosaccharides.
