The adenoviral vector, AdPG, was also shown to offer p53-responsive expression in prostate carcinoma cells LNCaP (wt p53), DU-145 (temperature sensitive mutant of p53) and PC3 (p53-null, but engineered to express temperature-sensitive p53 mutants).
The effectiveness of an insulation material is measured in terms of its heat-transfer rate, or U-value, often expressed as the number of BTUs passing through a given unit of insulating material each hour at an expressed temperature differential across the material.
A concept, specific temperature difference, is proposed which expresses temperature difference of unit area density and can estimate the overall thermal insulation performance of lightweight substrate.
The most clearly expressed temperature dependence of the electric resistance is observed for CM GNP/L285 with GNP concentrations of 0.023 and 0.034 vol.
The kelvin is used in science and in engineering, as it expresses temperature intervals better than other scales, which do not originate from absolute zero.
Similarly, Erg1 degradation was virtually blocked in cdc48-3 and npl4-1 cells, expressing temperature sensitive alleles in essential subunits of the Cdc48 ATPase complex that pulls substrates out of the ER membrane after Doa10-dependent ubiquitination.
The plasmids pCMVSport6humanCHC (expressing wild type clathrin heavy chain) and pCMVSport6humanTSCHC (expressing temperature-sensitive clathrin heavy chain) were transfected via electroporation into DKO-S cells [17].
hFOB1.19 cells (normal human fetal osteoblasts expressing temperature-sensitive SV40 large T antigen) were obtained from ATCC and cultured at the permissive temperature (33.5 °C) in a 1 : 1 mixture of Ham's F12 medium and Dulbecco's Modified Eagle's medium containing 0.3 mg/ml G418 and 10% fetal calf serum but lacking phenol red.
FDCP-mix cells expressing temperature-sensitive p210 bcr/abl (Pierce et al, 1998) were cultured in Fisher's medium, 20% v v−1 horse serum and 5% v v−1 murine IL-3 conditioned medium (CM) from the X63Ag8-653 myeloma cell line as a source of murine IL-3 (Karasuyama and Melchers, 1988).
It would be a lot more useful to express the temperature in Centigrade or Fahrenheit, because that would do a much better job of showing the temperature differences that actually matter to people.
