Sentence examples for express nada from inspiring English sources

In order to express NadA, the nadA gene was amplified by PCR from chromosomal DNA of Neisseria meningitidis MC58 using the primers NadA-MC58-sense (5'-CGGATCCCATGGGCAAACACTTTCCATCC-3', NcoI site) and NadA-MC58-anti (5'-CCCGCTCGAGTTACCACTCGTAATTGACGCC-3', XhoI site).

Western blotting using whole cell lysate and polyclonal mouse anti-NadA allele 3 antibody indicated that these isolates did not express NadA (Dataset 1).

In an unbiased approach, the complete library was expressed and NadA screened against it.

Therefore, CEACAM1 transfected cells or control transfected cells were infected for two hours with E. coli expressing either NadA or Opc or the parental E. coli strain at an MOI of 40.

I then enlisted the help of my two teenage daughters, May and Nada, to guide me through the world of Egyptian blogs, where young Egyptian men and women can express themselves with relative freedom.

Additionally, meningococci express a panel of proteins that not only mediate adhesion, but also promote invasion into host cells, such as colony opacity associated (Opa) proteins, Opc and NadA [11].

Figure 6: NadA binds CHO cells expressing hLOX-1.

The binding between LOX-1 and NadA was further corroborated by cellular binding assay in which the CHO cells transiently expressing LOX-1 were able to bind recombinant NadA.

However, we did not observe CEACAM1 binding by recombinant E. coli expressing the oligomeric, functional form of this protein suggesting that NadA is not the second CEACAM1-binding adhesin of N. meningitidis.

Although phase variable, NadA may contribute to bacterial virulence as it is expressed by ~50% of disease isolates compared with ~5% of strains isolated from healthy individuals [ 103].

NadA binding was observed selectively on the entire LOX-1 expressing cell population (Fig. 6, bottom right panel).