A cell line known to express messenger ribonucleic acid of adrenomedullin and its receptor was used to optimize the polymerase chain reaction and served as a positive control preparation in all experiments.
We found that the ST-2 cells in these cultures express messenger RNA (mRNA) encoding a repertoire of many of the reported osteoclastogenic factors (interleukins [IL]-1/IL-1R1, IL-11, IL-6/IL-6R, and IL-17 transformingrowthth factor [TGF]-β), as well as the recently described OC differentiation factor (ODF/TRANCE/RANKL).
The rapid turnover of highly expressed messengers is probably required for the rapid adaptation of cell physiology in a changing environment.
An overlapping set of 153 transcripts emerges when the 1328 predicted 63-set target mRNAs (3 or more sites with a TargetScan context score of 60th percentile) were compared to the 1806 differentially expressed messenger RNA transcripts identified in a SNL-L4 ipsilateral versus contralateral comparison.
Using next-generation sequencing, Brandley and colleagues obtained a read out of nearly all expressed messenger RNA in the uterus of a pregnant and the uterus of a nonpregnant lizard.
Protein kinase CK2 is a profoundly conserved, perpetually expressed, messenger independent and constitutively (presumably) active serine or threonine kinase that can use ATP as well as GTP as co-substrate localized in cell nucleus and cytoplasm.
We have previously used a subtraction screen on two cDNA libraries covering embryo development from early fertilization events (6-12 hours post-fertilization) to ovules bearing late torpedo stage embryos as a method to produce an EST pool enriched for weakly expressed messenger RNAs.
On June 13, the Massachusetts Provincial Congress was notified, by express messenger from the Committee of Safety in Exeter, New Hampshire, that a New Hampshire gentleman "of undoubted veracity" had, while visiting Boston, overheard the British commanders making plans to capture Dorchester and Charlestown.
We confirmed that mouse SN DA neurons predominantly express GIRK2 messenger RNA alone, together with D2 dopamine receptor messenger RNA (Fig. 3A, D2 receptor and GIRK2 were co-expressed in 100% of analysed SN DA neurons, n = 85, without other GIRKs in ∼80%).
We engineered T cells to transiently express a messenger RNA encoding a chimeric antigen receptor (CAR) specific for mesothelin, a protein that is overexpressed by PDAC cells.
