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One expression cassette design that has been used to express long sense and antisense RNAs in non-mammalian cell types is symmetric transcription using convergent promoters.
Here, we focused only on pol II expression of shRNA but an alternative approach would be to express long hairpin RNA (lhRNA) where the stem length is extended up to 300 bp [ 24- 26].
As a positive control for TE expression detection, we transfected human HeLa cells with a plasmid to exogenously express Long INterspersed Element 1 (LINE-1 or L1).
For example, vectors based around the 'full virus' strategy can now be used to express long polypeptides (at least 140 amino acids long) as fusions to the coat protein.
In his book, the cockroach cartoon serves as a vehicle for the Azeri to express long pent-up frustrations about economic and social marginalization.
ETEC express long, proteinaceous appendages or fimbriae on their surface, which mediate adhesion to the gut epithelium [ 4].
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WB of protein extracts from transduced NSC confirmed that both isoforms express long-term from the EF1α promoter, but expression from the MeP promoter is negligible until NSC are induced to differentiate (data not shown).
Even so, Mr. Deromedi and the analysts who follow the company, express long-term confidence.
The Kosovo war was a watershed that made it acceptable for Russian politicians and citizens alike to express long-concealed suspicions of the West.
The remaining six peripheral photoreceptors express long-wavelength-sensitive opsins.
Our results demonstrate that with thoughtful vector design one can successfully express long-term, therapeutic levels of virally encoded human β -globin in the erythroid progeny of hematopoietic stem cells.
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