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Salt samples were tested directly in field for iodine content using spot express kits.
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Because GFRα1 is specifically expressed in As, and Apr undifferentiated spermatogonia in vivo and, therefore, the GFRα1+ population did not express Kit [3], we also checked expression patterns of GFRα1 at the protein level by flow cytometry.
Some pediatric tumors express KIT (2, 23), but little is known about expression or the role of endothelial cell KIT in pediatric brain tumors (21).
They have intrinsic proliferative capacities and express Kit and EpoR at different levels.
We previously reported that a significant proportion of GS cells express Kit [13].
These findings contrast with previous observations that SSCs do not express Kit.
Moreover, a variable proportion of the cells express Kit, suggesting that a majority of GS cells are differentiating spermatogonia.
The cDNA was used as a template for subsequent in vitro transcription with biotin-labeled UTP at 37oC for 16 h using Genechip 31 IVT express kit (Affymetrix).
In some respects, our observation is reminiscent of the Clermont model of spermatogonial renewal, which proposes that A1 A4 spermatogonia, all of which express Kit, form a loop by recruiting a part of A4 into A1 [29], [29].
Based on these observations, we suggest that, when SSCs are relieved from steady state kinetics, such as after germ cell transplantation or in vitro culture, they may be exempted from required differentiation and are induced to express Kit (Figure 5).
hESCs did not express KIT-LIG and REX1.
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