Sentence examples for express inserted from inspiring English sources

To express inserted genes using Streptomyces species as the production hosts, an integration expression cassette is required to be inserted into the BAC vector, which includes genetic elements encoding a phage-specific attachment site, an integrase, an origin of transfer, a selection marker and a promoter.

As already stated, IR-carrying constructs are induced to move systemically and express inserted sequences following challenge inoculation with TYLCV.

The first way is based on direct expression, for instance, by detecting pigmentation or colony morphology (Brady 2007), both of which may directly result from the expressed inserted gene (Craig et al. 2010; LeCleir et al. 2007).

These reports, together with our currently demonstrated replication competence of HIV-1 genomes expressing inserted cellular miRNAs, encourage additional investigation into the nuanced miRNA-encoding capabilities of DNA viruses, RNA viruses, and retroviruses.

Two main hurdles can still be identified, i.e., the successful expression of genes in metagenomic clone libraries and the subsequent screening and selection of genes of interest from expressed inserts.

Hexokinase-encoding cDNA sequences from Physcomitrella were cloned into the high copy number 2 μm URA3 shuttle vector pFL61 [ 38], which expresses inserts in yeast from the constitutive PGK promoter (Additional files 2 and 3: Tables S2 and S3).

We also constructed vectors that fuse a fluorescent protein and the mitochondrial targeting sequence of Listeria monocytogenes ActA protein (Mito tracker) ([ 20]) to the COOH terminus of the expressed insert.

These retroviral genomes express the inserted miRNA and are generally replication competent in T-cells.

Next, we asked if chimeric NL4-3 miRNA viruses that express the inserted intragenomic miRNA support a spreading virus infection in cultured human cells.

In replicating mammalian cells expressing TVA, the viral vector can stably integrate into the DNA and express the inserted experimental gene at high levels, but the virus is replication-defective since viral RNA and proteins are inefficiently produced [79].

To date, while many studies have shown that lentiviral vectors containing miRNAs can transduce mammalian cells and express the inserted miRNA efficiently, no study has examined the impact on the replication of a lentivirus such as HIV-1 after the deliberate intragenomic insertion of a bona fide miRNA.