Sentence examples for express indicated from inspiring English sources

(A ) Isogenic SAS-6 -/-; p53 -/- cells carrying indicated SAS-6 constructs (see 'Methods') were arrested in S phase and induced to express indicated SAS-6 mutants for 16 hr and then immunostained with indicated antibodies to visualize de novo centrioles (centrin, green; SAS-6/HA, red).

(C, D ) Clone #2 acentrosomal cells were infected with lentiviruses, and induced to express indicated SAS-6 constructs for 16h (C ) or 3 days (D ).

(C ) Isogenic SAS-6 -/-; p53 -/- cells carrying indicated SAS-6 constructs (see 'Methods') were induced to express indicated SAS-6 mutants for 3 days, and then processed for BrdU pulse-chase before fixation for immunofluorescence.

Isogenic SAS-6 -/-; p53 -/- cells carrying indicated SAS-6 constructs (see 'Methods') were induced to express indicated SAS-6 mutants for 3 days, and then processed for serial sectioning electron microscopy.

(A C ) Isogenic SAS-6 -/-; p53 -/- cells carrying indicated SAS-6 constructs (see 'Methods') were induced to express indicated SAS-6 mutants for 3 days, and then processed for serial sectioning electron microscopy.

(B ) Isogenic SAS-6 -/-; p53 -/- cells carrying indicated SAS-6 constructs (see 'Methods') were induced to express indicated SAS-6 mutants for 3 days, arrested in G1 for additional 36 hr, and then processed for immunofluorescence microscopy to visualize cilia (GT335, green) and distal appendage (CEP164, red).

Purified GST-fusion constructs bound to Glutathione agarose beads were incubated with H4 cell lysate expressing indicated expression constructs.

(B) Confocal microscopic analysis of HeLa cells expressing indicated GFP or GFP-tagged Trak1 proteins (green) with mitochondria labeled by MitoTracker Deep Red (MitoT; purple) and nuclei by DAPI (blue).

(D) 3D-SIM imaging analysis of HeLa cells expressing indicated GFP or GFP-tagged Trak1 proteins (green) with mitochondria labeled by DsRed2-Mito (DR-mito; red) or anti-TOM20 antibody (red).

Transfections used Fugene-6 (Roche) and equal amounts of plasmids expressing indicated cDNAs in pcDNA3-based vectors co-transfected with equal amounts of pTK-hygro.

(B ) Immunoblot of worms expressing indicated transgenes using GFP antibody.