If activation-associated modifications could always spread over a long distance, many genes would be expressed inappropriately.
However, if our aim is to identify genes that are causally driving aspects of the neoplastic phenotype, then we need to identify those sequences which are expressed inappropriately as a consequence of induced DNA damage or erroneous epigenetic marking.
It provided a forum for cynics, and due to inexperience, some people expressed themselves inappropriately.
The result is as predictable as it is painful: That which is repressed will still be expressed, just inappropriately.
Globally releasing it could catastrophically affect the pattern of genes expressed by inappropriately activating most genes.
Mbd3-deficient ES cells have been shown to inappropriately express trophectoderm-specific genes in certain conditions (Kaji et al., 2006; Zhu et al., 2009).
However, some cancer cells inappropriately express N-cadherin, which promotes cellular motility and invasion (Islam et al, 1996; Nieman et al, 1999; Hazan et al, 2000; Derycke and Bracke, 2004).
Two previous studies observed that ttk mutant embryos inappropriately express the neuronal protein Futsch (Hummel et al., 2000), the antigen of the 22C10 antibody (Zipursky et al., 1984), in somatic and visceral muscles (Giesen et al., 1997; Murawsky et al., 2001).
This is plausible in light of findings that in daf-2 mutants, dauer-stage genes are inappropriately expressed in non-dauers [46]; presumably inappropriate repression of non-dauer-stage genes also occurs.
Such inappropriately expressed genes may be detected by microarray or other expression analyses of tumour against normal tissue.
