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Another negative correlation was observed between nuclear expression of pY530Src and ER status (χ P<0.001), suggesting that ER-negative breast cancer patients are more likely to express inactive Src in the nucleus, valued as the site of inactivation.
Compared to control flies, that is, flies that express inactive tetanus toxin UAS-Tet, Dv-E GAL4/UAS-TNT flies have a severe circadian locomotor activity deficiency.
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Therefore, at one week after initiation of sustained C-MYC induction, a hydrodynamic injection [1], [2] was administered containing no plasmid (saline only) or 20 µg each of a luciferase-expressing donor plasmid (pLiLucB) and plasmids containing vector backbone alone (pCS), expressing inactive integrase (pCSmI), or active wild-type integrase (pCSI) (Fig. 1b).
We generate stable MDCK cell lines expressing inactive (T22N mutant) and constitutively active (Q67L mutant) Rab13 as GFP‐Rab13 chimeras.
Nevertheless, melon and cucumber plants expressing inactive CsACS2 isoforms are not compromised in carpel formation, suggesting that other ACS isoforms provide the carpel-promoting function.
Recently a group reported that infection with an attenuated strain of B. pertussis, expressing inactive PT, protects mice against highly pathogenic influenza A viruses by dampening the cytokine storm [52].
In contrast to the cells transfected with functional CD44-CP1 (Figure 6B), cells expressing inactive Flag-CP1 showed no difference in the phosphorylation of TSC2 at S939 (Figure 6B), indicating that active, membrane-bound CP1 could protect TSC2 from its inactivating phosphorylation at this site.
(B ) Zymogram analysis of the activity of autolysins extracted from different Staphylococcus aureus atl mutant strains expressing inactive amidase (AMH265A), inactive glucosaminidase (GLE1128A), or both (AMH265A GLE1128A).
Macrophages from IKK1-deficient mice or knockin mice expressing inactive IKK1 show increased production of proinflammatory cytokines as a result of enhanced IKK2 activation and IκBα degradation [ 34].
ELISA absorbance values were reproducibly and significantly higher for samples from wild-type PglB strains compared to samples from isogenic control strains expressing inactive PglB.
In contrast, WT-p53 expression diminished TGF- β-mediated Nox4 oxidase activity comparable to cells that were untreated or cells that expressed inactive Nox4-DN.
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