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The significantly enriched GO associations annotated in various gene sets are present in additional files, genes constitutively expressed (Additional file 10), genes expressed in Five clusters (Additional file 11) and genes differentially expressed in pairwise comparisons (Additional file 12).

Interestingly, a substantial fraction of these novel TUs were differentially expressed (Additional File 1), including a TU that showed almost exclusive expression in degenerative embryos.

Several other genes belonging to the solute carrier family were also differentially expressed (Additional file 1).

From this analysis, 63 miRNAs were found to be differentially expressed (Additional file 1: Table S6).

Most mitochondrial genomic genes were selectively expressed (Additional file 1: Figure S8).

In contrast, nearly all T. urticae ABC fragments or pseudogenes were not expressed (Additional file 8).

All three genes were found to be almost ubiquitously expressed (additional file 2).

In all developmental stages of T. japonicus, the TJ-ABCH_88708 and TJ-ABCE1 genes are highly expressed (Additional file 10).

Genes having P-values with an FDR threshold of < 0.05 were designated as differentially expressed (Additional file 5).

PCR fragments were obtained for all of them indicating that these genes are truely expressed (Additional file 3).