He did not remember expressing it definitively.
However as the long form is definitively expressed and acts as an activator of hedgehog targets in response to hedgehog signalling (see below), we can conclude that midline-derived hedgehog signalling in amphioxus involves activation of ventrally-expressed target genes in ventral cells, and may also involve repression of these genes in dorsal cells, or indeed of other genes in ventral cells.
On the basis of these elements Zhu et al. express a certain skepticism in definitively ruling out the association between psoriasis, psoriatic arthritis, and NOD2/CARD15.
Finally, recent data have definitively demonstrated that human endothelial cells may express a previously unrecognized receptor for CXC chemokines named CXCR3B and derived from an alternative splicing of the CXCR3 gene [ 20].
As the average number of ESTs recovered per library was relatively low (few copies of ZnT sequence per library), we can not definitively conclude that tissues without ESTs for a given ZnT do not express this gene at all.
To address this issue definitively, several investigators have stable radiation chimeric mice in which bone marrow cells express enhanced GFP on the beta-actin promoter.
Germ cells are definitively recognized at ∼7.2 days post-coitum as an extra-embryonic cluster of cells that express tissue non-specific alkaline phosphatase, Oct4 and Stella, at the base of the allantois (12– 12).
Because the endogenous protein is not mosaically expressed, it is not possible to definitively resolve the expression to either the anterior or posterior face of the cells.
We conclude that c-KIT positive SEM, or at least tumors with simultaneously expressed c-KIT and PLAP can definitively be classified as CSEM.
Here we show more definitively that Foxc1 is expressed by brain pericytes and that it is essential for normal pericyte function in the developing brain.
To definitively identify the Foxc1 expressing cell type in the brain vasculature, we used markers of ECs (PECAM) and pericyte/vSMC (PDGFrβ, smooth muscle actin-α) in conjunction with Foxc1 immunostaining.
