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The results indicate that FPV recombinants can express correctly the E6 and E7 oncoproteins, and they should represent appropriate vectors for the expression of these oncoproteins in human cells.
Attempts to express correctly folded and localised BesB using N- and C-terminal his- and strep-tags as well as fusions with maltose-binding protein and GFP were repeatedly unsuccessful.
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As these quantifications ignore the size of the transduced area which directly affects the absolute number of positive cells per section, we quantified the density of cones expressing correctly the GNAT2 marker in the region of wt RPE65 expression.
Indirect immunofluorescence assays in transfected HeLa cells indicated that the recombinant DNAs (rDNAs) could be expressed correctly and had antigenicity.
It is shown that the photocathode life time cannot be expressed correctly in the units of the collected anode charge.
You have to be absolutely sure the fiber optic was in the right place and that the protein was expressing correctly," says Post.
One bottleneck for studying membrane proteins is the difficulty in expressing correctly folded and stable proteins, which often requires extensive protein engineering and multiple rounds of optimization, a time and resource intensive process.
After verifying that all mutant PB2 proteins were expressed correctly (Fig. S1), their nuclear import in HeLa cells was tested by indirect immunofluorescence using PB2-specific antibodies.
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These results show that αReps are expressed, correctly folded and bind specifically their target in eukaryotic cells.
First, the decreased amplitude could reflect a decrease in the number of channels that are expressed correctly in the membrane as compared to wild type.
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