Sentence examples for express competent from inspiring English sources

For protein expression, plasmids were transformed into T7 express competent Escherichia coli BL21 (DE3) cells (New England Biolabs) according to the manufacturer's instructions, and transformants were selected on LB plates containing ampicillin (100 μg/mL).

The pGEX-T2 plasmid containing the gene encoding for Bxe_B3018 was transformed into Arctic Express competent cells.

Proteins were expressed in T7 express competent E. coli cells (NEB) and purified using Ni-Sepharose Fast Flow (GE Healthcare) and Amylose resin (NEB) according to standard protocols.

Melanoma cells expressing transcriptionally competent and constitutively active HSF1 mutant characterized by an enhanced expression of HSPs did not acquire resistance.

After reporting that cultured lung cancer cells and tissues possess the capacity to express both catalytically competent and non-competent AChE molecules [ 8] insights into possible differences in size and abundance of active and inactive AChE subunits were gained by western-blotting of unaffected and cancerous tissue extracts using N19 antibodies.

All tau cDNAs in pET30a were expressed in competent BL21 (DE3) cells after growing to an OD (600) of 0.5 with 0.5 mM IPTG induction for 2.5 hours.

These data indicate that the GHR, which is expressed and competent during the time of the postnatal leptin surge, mediates the developmental actions of GH on ARH projections.

Preferences expressed when competent may change as cognition declines, giving rise to complexities with regards to ascertaining what the true preference for care may be.

While histone acetylation genes are found at lower levels (Additional file 4) and histone deacetylases (sirtuins) are highly expressed in competent larvae, the opposite is true after settlement.

The GST-tagged fusion protein of the phosphatase domain of human PHLPP1 (Pro 627 Pro 990, PHLPP1α numbering) or PHLPP2 (Gly 745 Asn 1102) was expressed in competent BL21 DE3 pLysS cells and purified according to the method previously described.

The European Landscape Convention (2000) obligates European Union countries to identify and implement landscape quality objectives (LQOs) understood as the specification of public expectations and preferences concerning the landscape of a given area, expressed by competent public authorities.