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The constructed plasmids pNprB-bglB (which attempted to express bglB with promoter Peno and signal peptide NprB) and pbglB (which attempted to express bglB with promoter Peno) (Fig. 1) were also not successful, except the present signal peptide SP1086.
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Construction of plasmids pNprB-bglB (express β-glucosidase gene bglB with promoter Peno and signal peptide NprB), pbglB (express β-glucosidase gene bglB with promoter Peno), pZM1086 (express ZMO1086 gene with promoter Peno), pSP1086-bglB (express β-glucosidase gene bglB with promoter Peno and signal peptide SP1086), and pSacB-bglB (express fusion protein SacB-bglB with promoter Peno).
Even higher amounts, up to 9.3% TSP, could be achieved by the expressing of BglB cellulase from T. maritima in the chloroplast of tobacco using a modified alfalfa RbcsK-1A promoter and a transit peptide [ 140].
"I express myself with images".
People express frustration with the constant testing.
Bosnian Serbs often express solidarity with Serbia.
To express itself with language.
Never express exasperation with a crying woman.
Musicians express similar enthusiasm, with less formality.
(Isorna et al. 2007) purified a β-glucosidase, named as BglB, produced by P. polymyxa and obtained the crystallographic structure of the BglB with glucose.
Another method for enhancement of the β-glucosidase secretion is to fuse the β-glucosidase encoded by bglB with the levansucrase encoded by sacB from Z. mobilis ZM4 to achieve the secretive expression.
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