Western blot analysis demonstrated that endogenous Brk expression levels were reduced, while wt- and km-Brk (both flag-tagged) were expressed appropriately in either HaCaT or MDA-MB-231 cells; total ERK5 levels remained similar throughout these manipulations.
By her standards I am absolutely unequivocally FAT (expressed appropriately in capital letters and always uttered with disdain).
As long as the transgene is expressed appropriately, calculating the copy number is not usually performed.
We first investigated whether components of this pathway are expressed appropriately to influence vascular development at the LPM.
Nevertheless, rhombomeric organization does arise in the mutant hindbrain and some rhombomere markers are expressed appropriately while others are reduced or lost.
These proteins can heterodimerize and SPT can complement dehiscence defects in alc mutants if expressed appropriately, although the converse is not true [ 18].
In the chick, the FGF7 subfamily members Fgf3 and Fgf10 are expressed appropriately, confined to the forming NH when pioneering H-NH axons extend.
Some patient-important outcomes, such as quality of life or symptoms, cannot be expressed appropriately as binary outcomes without substantial loss of information.
With the knowledge that LAMP1 YCaM was stable, expressed appropriately and targeted as predicted, we next examined its responses to various stimuli designed to liberate Ca2+ from intracellular stores.
Consistent with this, we found that colIIa was expressed appropriately in the posterior neurocranium of ethanol-treated mutants, although, as expected from the phenotype, no ethmoid plate or trabeculae condensations were present (supplementary material Fig. S4).
This may have been the case with Fragment 3, a fragment for which there was no significant seroreactivity, suggesting that it may not have been expressed appropriately, particularly given that previous work has found that the two VAR2CSA domains included in this fragment, DBLpam3 and DBLpam4, were seroreactive in multigravid east African women.
