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As with other anticancer drugs, TNBC can develop an acquired resistance after repeated exposure to PAC.
As expected, meristem size was reduced in wild type upon exposure to PAC.
The colony number was reduced by exposure to PAC, DS or Cu alone.
In contrast, the clonogenicity of BC cell lines was significantly inhibited by DS/Cu and totally eradicated by exposure to PAC plus DS/Cu.
After 24 h exposure to PAC at 25 n M for MOS or at 10 n M for LM8 cells, the percentages of cells in the G1 and S phases decreased and those in the G2/M and sub-G1 phase increased.
After 16 h exposure to PAC (40 n: 4 18-fold higher than IC50 concentration), DS (200–250 n: sub-IC50 concentration)/Cu1 μ or PAC and DS/Cu in combination, the treated cells were collected and cultured in drug-free medium for 7–14 18-fold
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Quantitative gene expression analyses indicated that exposure to PAC-treated dentin increased the expression of key biomineralization and odontogenic differentiation regulators, including RUNX2, BMP2, OCN, and DSPP.
Using the Calgary device (consisting of inverted plastic pegs that hang down into the media of a 96 well plate, to which bacteria attach and form biofilm), exposure to PACs showed a 49.5% decrease in bacteria attached to the inverted pegs as compared to control at 100 μg/mL (p < 0.1), but no statistical difference at any other concentration, confirming the preformed biofilm results above.
The cytotoxicity of csMSN, PAC, and PAC-csMSN was measured in cell monolayers by exposure to csMSN, PAC, and PAC-csMSN suspension at different concentrations for 72 h.
But in response to the new legislation, Procter & Gamble argued that "colour does not play a critical role in a child's accidental exposure to laundry pacs".
But in response to the new legislation, Procter & Gamble argued that "color does not play a critical role in a child's accidental exposure to laundry pacs".
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