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The presence of aggregates in exposure suspension is well documented [Gilmour et al., 2004; Bourdon et al., 2012].
The amount of carbon agglomerates in exposure suspension increased by decreasing particle concentration and may be an artefact caused by inefficient DLS measurements at low concentrations of dispersed particles.
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During exposure, the suspension was gently stirred and cooled.
As was the case in confluent cultures, involucrin was not induced in SCC4 cells during hypoxic exposure in suspension culture.
After exposure, the suspension was filtered through a 0.22 μm poly ether sulfone) membrane (Genesee Scientific) to separate the doxorubicin-loaded hydrogel-coated nanoshells from the free doxorubicin in the sample.
After plasma exposure, cell suspension was divided into 96-well tissue culture test plate (30096, SPL; 1 × 10 cells per well) for cell number counting and mitochondrial enzyme activity.
Laser exposure of suspensions of nanoparticles in liquids leads to excitation of high energy levels in both liquid and nanoparticle material.
This study aimed to investigate molecular and biochemical effects in roach (Rutilus rutilus) during a 10 days exposure to suspensions of three natural sediments that differed in the level of DLC contamination.
For the in vitro exposure, cell suspensions were incubated for 1 h with the same series of drugs.
Exposure to suspensions of nanoparticles was used to gauge the range of responses of this cell line to the silver and nickel oxide nanoparticles.
Thirty minutes after exposure, WBC suspensions (5 × 10 cells per sample) were fixed with 10% formaldehyde (Fisher Scientific, USA) and incubated for 10 min at room temperature.
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