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After phosphor-screen exposure, slides were counterstained with Nissl stain and dehydrated through a graded series of ethanol and xylene.
Scanning of the DDVP exposure slides, was performed using an Axon GenePix 4200 AL scanner with GenePix® software (Molecular Devices, Union City, CA) with a scan resolution of 5 μm, standard green filter, PMT gain set at 400 and scan power set to 30%% (first) then 100%% (second).
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The painted words "People die of exposure" slide down the pristine gallery wall.
Slides with T47D/PRL clones, control cell lines and anterior pituitary tissue were developed after 1 2 weeks of exposure, whereas slides with placenta, breast carcinoma tissue and breast cancer cell lines were developed after 6 8 weeks of exposure.
The dose distance relationship, for example, was based until 2015 on a small dataset on pollen deposition derived from exposure of slides near to a single maize field for 7 days [19].
mRNA signals were revealed by exposure of slides to autoradiographic film or by development in photo-emulsion for microscopic inspection.
For each exposure, the slides were placed inside a box along with the ozone generator.
After microwave exposure, the slides were allowed to cool to room temperature.
After exposure, the slides were developed in Kodak D-19, washed in distilled water, and fixed in Kodak rapid fixer diluted 1 1 with distilled water.
Following the initial exposure, the slides were emulsion-dipped (NTB; Kodak), dried and stored in a desiccated, lightproof container for 14 days.
After exposure, the slides were developed in Kodak D-19 (Carestream Health, Inc., Rochester, NY), washed in distilled water, and fixed in Kodak rapid fixer diluted 1 1 with distilled water.
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