Micro-CT scans were performed using a bench-top scanner (explore Locus, General Electric Health Care, London, ON, CA) with the following parameters: 210 views over 1° increments, 5 exposures averaged per view, 400 ms exposure time using 80 kV and 0.45 mA, isotropic reconstructed voxels with 0.045 mm dimensions, scan time approximately 17 minutes.
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CT images were acquired with an integrated CT scanner using an X-ray tube at 45 kVp and an exposure time of 1,000 ms per view.
CT scans were performed with the integrated CT using a tube voltage of 45 kV and an exposure time of 1,500 ms per view.
The individual organ specimens were staged in a high-resolution µCT scanner (Scanco µCT40, SCANCO USA, Southeastern, PA) and scanned at 10 µm resolution, 2000 views, 5 frames per view, 300 ms exposure time, 55 kVp, 144 µA.
Images were acquired in list-mode with the following parameters: 50 kVp X-ray source voltage, 180 μA current, a composite X-ray filter of 0.5 mm aluminium, 120 ms camera exposure time per projection, 9 projections per view, 23 × 35 mm field of view, acquiring projections with 0.7° increments over a total angle of 180°, producing images with a real pixel size of 35 μm.
The image data were acquired at 6 μm isometric voxel resolution with 300 ms exposure time, 2,000 views, and five frames per view.
