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We tested calreticulin exposure on the outer cell membrane of Tc cell-treated, ECTV-infected target cells.
To examine if the mode of induced cell death was apoptosis, macrophage monolayers were incubated with a range of supernatant volumes and assessed for detection of Phosphatidylserine exposure on the outer leaflet of the cell membrane by annexin V binding.
The cell membrane remains intact, although the change in membrane polarity leads to phosphatidylserine exposure on the outer membrane.
Eryptosis shares several key features with apoptosis of nucleated cells, such as cell shrinkage, membrane blebbing, and phosphatidylserine (PS) exposure on the outer cell membrane.
Turnover of erythrocytes can be triggered by eryptosis, which can be detected by PS exposure on the outer leaflet of the cell membrane.
(3) Prediction of tyrosine exposure on the outer side of A. thaliana microtubule wall and experimental verification of microtubule phosphorylation on tyrosine in cells.
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Later on, the outer narthex was constructed.
After exposure to hydrogen peroxide (H2O2) or acetic acid, yeast cells exhibit DNA strand breakage, chromatin fragmentation and exposure of phosphatidylserine on the outer plasma membrane [4] [6].
This asymmetry is maintained by active transporters (Devaux, 1992; Suzuki et al., 2010), but is lost in cells undergoing apoptosis or microvesiculation, leading to the exposure of PS on the outer membrane (Zwaal et al., 1977).
XPS/SRPES analysis, combined with operando X-ray Absorption Near Edge Structure (XANES) and AFM measurements, indicated that water induces severe aggregation of CeO2 nanoparticles, exposure of CePO4 on the outer layer of the aggregates and incorporation of the catalytic-active Pd nanoparticles into the bulk.
One of the earliest changes in cells undergoing apoptosis is the exposure of phosphatidylserine on the outer membrane leaflet.
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CEO of Professional Science Editing for Scientists @ prosciediting.com