The effects of RDX exposure on transcript expression in the eukaryotic components of the holobiont (coral animal and algal symbiont) were assessed simultaneously by investigating quality-assured RNA [ 32] collected from whole corals exposed in the exposure bioassay.
Using this higher-throughput design, we were able to examine the time-course (1, 3, 6, 9, and 24 h post-exposure) of transcript production and cytotoxicity in well-differentiated human bronchial cells exposed to diesel particulate matter at levels of 'real-world' significance.
After 10 days of exposure to CFA, transcript levels of all hepatic biotransformation genes were significantly elevated at one or both treatment levels compared with the respective control, with the exception of mdr1.
However, while hsp70 level decreased by a small extent by 30 days of exposure, hsp110 transcript level abruptly decreased, probably to nullify the negative effects of its expression at high level or as its role is substituted by some other chaperone over time.
However, after 10 days of exposure, cyp27a transcript levels were significantly increased at both exposure concentrations; the opposite of what might be expected.
After 4 days of exposure, pparα transcript levels were significantly higher in the fish exposed to 20 mg/L compared with the control animals (approximately 2.5-times control levels).
The impact of BPA exposure on ERβ transcript was examined further in the LAPC4 prostate cancer cell line, which harbors wild-type AR and is androgen dependent.
In the case of Ddit4 and Arl2bp, over-expression of miR-153 after ethanol exposure, resulted in the predicted reversal of transcript expression to control levels (p<0.0002 and p<0.027 relative to ethanol exposure, respectively).
This has been shown to manifest 24-h after exposure to hypoxia by increased expression of transcript and protein of COX4I2 when the related COX4I1 protein, being encoded on a different gene, is degraded (Fukuda et al. 2007).
Following 4 days of thermal exposure, hsp90 transcript level increased by 29-fold and then decreased to 5-fold relative to control as the exposure period was extended for 30 days.
Twenty-four hours post-exposure, the expression of transcripts was assessed using Illumina bead array technology and these responses were compared to non-irradiated controls.
