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In all cases, absence of O-PS correlated with exposure of the outer core (Figure 4, left panel) and the lipid A disaccharide (Moab Bala-1; not shown).
Tooth external surfaces were cleaned by wiping with sodium hypochlorite solution (6% w/v) before being encased in dental alginate; this facilitates specimen handling while also preventing exposure of the outer contaminated surfaces to either the laboratory or personnel.
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To examine if the mode of induced cell death was apoptosis, macrophage monolayers were incubated with a range of supernatant volumes and assessed for detection of Phosphatidylserine exposure on the outer leaflet of the cell membrane by annexin V binding.
(3) Prediction of tyrosine exposure on the outer side of A. thaliana microtubule wall and experimental verification of microtubule phosphorylation on tyrosine in cells.
The samples were washed with phosphate-buffered saline (PBS) and using Annexin V-fluorescein isothiocyanate (FITC) and PI staining for determination of phosphatidylserine exposure on the outer plasma membrane.
Ca2+-dependent PtdSer exposure on the outer leaflet of the plasma membrane was measured by confocal imaging of two PtdSer probes, either LactoglobulinC2 fused to Clover fluorescent protein ('LactC2') or Annexin-V conjugated to AlexaFluor-568 ('Annexin-V') (Shi et al., 2006; Hou et al., 2011; Kay and Grinstein, 2011).
Turnover of erythrocytes can be triggered by eryptosis, which can be detected by PS exposure on the outer leaflet of the cell membrane.
The ecto-CRT exposure occurs before the specific morphological, that is, phosphatidylserine (PtdS) exposure on the outer leaflet of plasma membrane, and biochemical, that is, mitochondrial transmembrane potential depolarization, apoptotic signs [ 71 ].
We tested calreticulin exposure on the outer cell membrane of Tc cell-treated, ECTV-infected target cells.
Eryptosis shares several key features with apoptosis of nucleated cells, such as cell shrinkage, membrane blebbing, and phosphatidylserine (PS) exposure on the outer cell membrane.
We have also previously shown that acetate induces apoptosis in CRC-derived cells in a dose-dependent manner, characterized by DNA fragmentation, caspase-3 activation, phosphatidylserine exposure to the outer leaflet of the plasma membrane and the appearance of a sub-G1 population.
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