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To quantify the expression of activated caspase-3 after drug exposure, flow cytometry was performed using an antibody against active caspase-3 (BD Biosciences).
In order to establish the nature of the cellular changes induced by HYAL exposure, flow cytometric analyses were performed using hematopoietic and non-hematopoietic markers on the four sub-populations described above.
In this way, the discontinuously generated smoke was diluted and converted into a continuous aerosol flow (cell exposure, flow rate of 5 ml/min and exposure of bacteria, 10 ml/min) which was sucked via negative pressure from a vacuum pump through the CULTEX® RFS module shown in Fig. 1 (CULTEX® Laboratories, Hannover, Germany).
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When exposure to flow was analyzed with respect to hole location, there was a significant difference due to the effect of flow and added exposure time (18 h) at hole four (P < 0.01, Fig. 9b).
The effect of upstream shear forces on platelet activation was assessed by quantifying P-selectin, integrin αIIbβ3, lysosomal glycoprotein, and phosphatidylserine exposure using flow cytometry.
We examined in vitro endothelialization of such tissue and retention of endothelial cells after exposure to flow resembling the in vivo situation.
The investigations were based upon three major factors that make the differences between original powder and its recycled powder: particle size, humidity exposure and flow additive concentration.
These findings indicate that changes to biomechanical properties in coculture and/or exposure to flow are correlated with changes in the cortical stress fiber density.
Interestingly, as observed in the companion study, cell seeding density exerts a profound influence on the local mechanical milieu of cells exposed to controlled fluid drag-induced shear stresses, which appears to relate directly to the mechanoadaptation response observed after exposure to flow.
A significant upregulation of miR-126 during long-term exposure to flow was shown.
These changes were reflected by significant upregulation of miR-126 during long-term exposure to flow.
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