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With the dissection and exposure completed, open visualization via the intraoral approach is used to divide the orbital septum.
After the exposure completed, cells were harvested in ice-cold phosphate buffer saline by scraping and washed with phosphate buffer saline at 4 °C.
After the exposure completed, were washed twice with HBSS before being incubated in 1 ml of working solution of DCFH-DA for 30 min at 37 °C.
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After 24 hours of LT exposure, complete MKK1-7 (except MKK5) deficiency was observed in lung epithelial cells, which was maintained over the observation period of up to 4 days.
2D:4D has also been shown to be sexually dimorphic in endocrine models of elevated (congenital adrenal hyperplasia) and reduced fetal testosterone exposure (complete androgen insensitivity syndrome).
Following exposure, subjects completed a paper and pencil questionnaire to assess their impressions.
After treatment exposure was completed, the media in the wells were aspirated and washed with PBS before they were replaced with another fresh media prior to experimental treatments.
Seventy-five adults with longitudinal assessments of cannabis use (60 with reported use, 15 with no reported use) and prenatal drug exposure assessment completed a spatial WM task during fMRI at age 28.
Participants consisted of 167 mother infant dyads participating in an ongoing longitudinal study of prenatal cocaine exposure, who completed the arm restraint procedure at the 7-month assessment (87 cocaine exposed, 80 non-cocaine exposed).
This arsenite As ratio hydride generation data were obtained after the animal exposure was completed.
Trained physicians who were blind to participants' As exposure status completed a comprehensive physical examination (Ahsan et al. 2006a).
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