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In this assay, Artemia nauplii are exposed to test compound and lethality is used to estimate cytotoxicity.
To determine the osmotic tolerance limits of iPSCs, cells were exposed to test solutions, which included varying concentrations of hypotonic buffer and hypertonic sucrose, for 15 mins.
L1 stage worms were exposed to test bacteria on NGM agar plates for 48 hours at 20°C before examination.
For EAG recordings, the head of each 4 8 day old control or dsRNA-injected female A. gambiae was excised at the occipital opening and placed on the reference glass electrode containing Hayes mosquito Ringer solution [51], mounted in a humidified air-stream (90 98% RH) and the antennae exposed to test ligands eluting from a gas chromatographic (GC) column [52].
Control cells were exposed to test medium supplemented with 0.2% ethanol.
mESCn were exposed to test compounds after 6 days of differentiation.
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Haskell Program Coverage (HPC) [51] was used to determine which areas of the source code and boolean controls were actually exposed to testing.
The cells were exposed to tested compounds for 3, 6, 24, and 72 h, washed with PBS, and then analyzed by measuring the half maximal inhibitory concentration of cell proliferation.
During the trial, fish were regularly and gently repositioned to ensure that they would all be exposed to testing conditions and would not evade from the high speed outer portion of the swimming ring.
They were sent up with early space flights to see how human cells coped with zero gravity and exposed to radiation to test the effects of nuclear weapons.
Next, cells were exposed to the tested compounds with concentrations ranging from 2.5 to 20 μΜ for 48 h.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com