Sentence examples for exposed to secondary from inspiring English sources
Aggressive management of septic shock clearly resulted in improved early survival in cirrhotic patients, who then become exposed to secondary infectious and non-infectious complications [37, 38].
However, samples exposed to secondary drying at 20°C had approximately 1.8% residual water.
On the next day, after 3 washes in PBS, the cells were exposed to secondary antibodies at RT for one hour.
After incubation, the membranes were exposed to secondary antibody (1∶1000 dilutions) and developed by nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP).
As a negative control, IE were incubated both with sera from non-immunized animals and without animal serum and then exposed to secondary antibodies specific against IgG from different animals spec. Data from 5000 infected cells (ethidium bromide positive) was collected on a FC500 flowcytometer (Beckman Coulter).
After multiple PBS rinses, the slides were exposed to secondary antibodies.
CFU produced by cells exposed to higher secondary drying temperatures were not statistically different from those removed from the lyophilizer after secondary drying at 5°C.
The membranes were then blocked overnight at 4°C, and after 2 h of incubation with the corresponding primary antibodies, membranes were washed and exposed to the secondary antibodies for 1 h at room temperature.
After incubation with conjugated LIBS or control single-chain antibody, platelets were exposed to a secondary antibody (Penta HIS Alexa Fluor 488, Qiagen, Hilden Germany) which selectively binds the histidine-tag of the single-chain antibody constructs, and flow cytometry was performed gating 10 000 platelets using a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).
As a control, two preparations were exposed to the secondary antibody without the primary anti-CB1 antibody.
Other individuals who are exposed to reclaimed secondary wastewater, including spray irrigators and people living near spray irrigation sites, could be potentially exposed to MRSA and MSSA.
