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We measured the amplitude of these "spontaneous" sleep-like waves, as the preparation was exposed to fixed durations of illumination.
For experiments examining effect of Indo dose effect, data were normalized against turbidity measurements of lipids initially exposed to fixed amount of triton X-100 but without Indo.
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Cells were then exposed to fixing solution consisting of 1% CaCl2 and 0.5% formaldehyde in milli-Q water for 2 min followed by two washes with 1% acetic acid and 50% ethanol in milli-Q water.
The analysed processes and scaling are: (a) In a process of reaction and diffusion inside a catalyst with fractal external surface exposed to a fixed reactant concentration the overall reaction rate scales as k(D/k)(d−Df /2, where D is the diffusion coefficient, k is the rate constant of a first-order reaction and d is the dimension of the embedding Euclidean space.
Additionally, we tested the ability of increasing Indo concentrations to alter the detergent resistance of DPPC or DOPC exposed to a fixed triton X-100 level.
Our purpose was to obtain efficacy and safety information from a cohort of subjects exposed to latanoprost/timolol fixed combination (FC) for ≥18 months using a prospective, observational design.
When participants are exposed to a fixed time lag between auditory and visual stimulation for 3 min, they subsequently show shifts in the time required between auditory and visual stimuli in order to perceive them as simultaneous, known as the "point of subjective simultaneity".
The cells were exposed to trypsin, washed, fixed with ethanol, stained with propidium iodide and analyzed by flow cytometry.
In brief, 5 × 10 cells were exposed to celastrol and fixed with 70% ethanol at −20 °C overnight.
To see if this finding could be confirmed by a different assay, the intact cells that were similarly exposed to ouabain were fixed and stained with an anti-PIP3 antibody.
Third, confocal imaging of FM1-43FX enabled the identification of large serpentine-like structures in beta cells (Fig. 6d) that had been exposed to carbachol and fixed within the lifetime of the large events determined by the two-photon SRB imaging experiments (Fig. 5c,e).
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