The membranes were washed and incubated in a luminol/peroxide detection solution from the SuperSignal West Pico Chemiluminescence Substrate Kit Piercee) for 5 min in the dark, exposed to Kodak Biomax ML film, and developed on a Kodak X-OMAT M20 Processor.
The membranes were wrapped in plastic wrap and placed in a film cassette in the dark and exposed to x-ray film (ECL hyperfilm, GE Healthcare) for 30 seconds to 2 minutes and developed.
Cotyledons were collected from 5 day-old seedlings either kept in the dark or exposed to light.
Cells were kept in the dark or exposed to blue light for additional 4 hr, and then analyzed for Fe-S cluster association.
DOI: http://dx.doi.org/10.7554/eLife.07597.039 10.7554/eLiFigure97.040 Figure 6 source data 3. Directionality values of Col-0 and phot1/2 plants grown with the root system in the dark or exposed to light in the top third of the rhizotron.
The reproducibility between biological replicates was excellent for cultures grown in the dark or exposed to light for 15, 60, and 120 min (R > 0.95); there was more divergence between the replicate cultures exposed to light for 240 min (R = 0.75).
Swatches with a 0.5% concentration of bacteria were exposed to light of 2000 foot candles or dark for a given period of time.
But every year, as the 24-hour polar night shifts to 24-hour summer sunlight, more than half the pack ice melts, and when that white ice changes to dark, open water, the exposed ocean, instead of reflecting the sunlight, absorbs it and begins to warm the overlying air.
The objective of this study was to design a pigmented coating with dark appearance that maintains a low temperature while exposed to sunlight.
With the same oxygen availability and temperature, the sample kept in dark had the lower increase in PV in shrimp oil, compared with that exposed to light (p < 0.05).
