Sentence examples for exposed to a secondary from inspiring English sources
After incubation with conjugated LIBS or control single-chain antibody, platelets were exposed to a secondary antibody (Penta HIS Alexa Fluor 488, Qiagen, Hilden Germany) which selectively binds the histidine-tag of the single-chain antibody constructs, and flow cytometry was performed gating 10 000 platelets using a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).
Slides were then exposed to a secondary goat anti-mouse antibody (1 : 500 in PBS) conjugated with Alexa-488 (Molecular Probes) and incubated for 1 h in the dark room-temperature-humidified environment.
Following a resting time of 5 days, a period during which monocytes in the absence of any primary event differentiate into macrophages, the cells were exposed to a secondary stimulation with either LPS or Pam3CSK4 (second stimulus) (Fig. 1A).
Membranes and plasma were incubated overnight at 4°C, washed three times in 1× TBS, and then, exposed to a secondary alkaline phosphatase-conjugated goat anti-human Fc antibody (Zymed, South San Francisco, CA) diluted 1 5,000 in blocking buffer.
The tissues were incubated with the anti-FSTL1 antibody (1 400 dilution) overnight at 4°C after blocking and then exposed to a biotinylated secondary linking antibody (Boster, Wuhan, China) for 20 minutes.
Blots were exposed to a horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) with development using enhanced chemiluminescence detection (ECL Kit, Amersham Pharmacia Biotech, Chandler, AZ, USA).
After incubation with the primary Ab, the array was then exposed to a biotinylated anti-mouse secondary Ab for 30 minutes.
On the other hand, no such spreading can be seen for the samples where a pre-adsorbed H*Protein layer has been exposed to a solution of any secondary protein.
The membranes were then exposed to an anti-rabbit secondary antibody (NA934; Amersham Pharmacia Biotech, Little Chalfont, UK; 1 5000 dilution).
Because of its larger duration, each secondary call is exposed to a larger number of interruptions.
Vizualization of the reaction product was accomplished using a biotinylated secondary antibody exposed to an avidin and biotinylated horseradish peroxidase macromolecular complex.
