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Exposing spheres for 11 days to differentiating conditions (DM) results in attachment of the spheres to the slide increased CK-19 positivity (35% positive cells; Figure 3D).
After initiating differentiation, we exposed the spheres to 10 μM BDE-47 or BDE-99 with or without 3 nM T3 or 1 μM NH-3.
When the user pulled a pin on the handle of the grenade, the casing would fall away and expose the sphere; another pin would activate the firing mechanism, and the user would then attempt to attach the grenade to an enemy tank or other vehicle, ideally with enough force to break the glass ball.
CFE of arsenite-exposed sphere cells was markedly elevated compared with adherent arsenite-exposed cells or either adherent or sphere control cells.
The cells driving tumor formation were present only in arsenite-exposed spheres, because when sphere cells were first separated from adherent cells and then inoculated into mice, only arsenite-exposed sphere cells rapidly produced highly pleomorphic cancers.
After separating floating and adherent cells, secreted MMP-9 activity from arsenite-exposed sphere cells was approximately 6 times that of either control (sphere or adherent) or arsenic-exposed adherent cells.
The local distribution of deposited filling gases (CO2) within a sample consisting of a packed bed of ∼1 μm-sized solid glass spheres exposed to a temperature gradient at cryogenic temperatures was investigated by neutron radiography measurements.
Spheres exposed to 1 μM MeHgCl for 48 hr served as positive controls.
As depicted in the graph, the increased expression of the differentiation marker CK-19 coincides with a decreased expression of the early progenitor marker PDX-1 and the β-cell precursor marker Isl-1 in spheres exposed to DM.
Continue until you have a (near) perfect sphere of exposed pink grapefruit flesh.
When 1.3 mm-diameter α-TCP spheres were exposed to an acidic calcium phosphate solution, brushite (dicalcium phosphate dihydrate) was formed on the surface of the α-TCP spheres.
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