Suggestions(1)
Exact(3)
From this table, we observe that when predicted probability is above 0.8, both the predicted buried label and exposed label could reach about 0.9 accuracy.
After incubation at 37°C for the indicated time, cells were rapidly chilled using pre-cooled PBS at 4°C, and moved to the coldroom to arrest endocytosis and remove surface exposed label or transferrin as described previously.
Remaining surface exposed label was removed by incubating the cells 2 × 10 min in cold 100 mM MESNa in MESNa buffer (MESNa buffer: 50 mM Tris, 100 mM NaCl, 1 mM EDTA, 0.2wt/vol BSA, pH 8.6) Finally, transferrin, if used, was removed from the plasma membrane by acid wash at 4°C (2 × 2 min with 150 mM Glycine, pH 3.0).
Similar(57)
Additionally, the GFP line allows us to expose labelled individuals to different social or environmental conditions over an extended period of time, and to assess relevant traits, and their reaction norms, of a given individual or genotype.
M0t can be determined from samples exposed to label long enough to ensure turnover of the entire pool, or using mass isotopomer distribution analysis (MIDA) (Hellerstein and Neese, 1999 ).
Surface exposed Ret labeled by pHluorin (surface-stained with anti-GFP antibody) showed even labeling of the entire dendritic tree, while the mCherry signal displayed a more granular distribution of cellular Ret.
This paper utilized data from two studies to explore whether consumers exposed to labels on genetically engineered foods expressed greater aversion to genetic engineering than consumers in control groups, who were exposed to decoy labels unrelated to the technology.
Since we are concerned here with wild type E. coli metabolome, we considered only the spectra where E. coli cells had not yet been exposed to labeled glucose (spectra acquired at time t = 0).
Western blot analysis showed that total IgG purified from a plasma pool of malaria exposed multigravidae labelled a single dominant band of 37 kDa in 1 mM IPTG induced bacterial extract and in purified DBL5ε (Figure 4B).
Susceptible hosts exposed to labelled spores become infected, suggesting that the dye does little or no harm to the spores (data not shown).
Concomitant osteoarthritis in the remaining sites may confound the associations between labelling in a specific site and quality of life: a subject may be included in the analysis as exposed to labelling regarding one joint and not exposed for the remaining joints.
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