Upon stress, these domains could expose interaction sites that promote assembly into amorphous aggregates.
The fact that we were more successful in finding binding partners using portions of the coding region in comparison with the full-length proteins raises the possibility that fragments might expose interaction domains that are otherwise impossible to expose in a yeast system, especially if certain cell-specific modifications are required to uncover such surfaces.
Setting aside the question of the optimal term used for these processes, in our framework we are able to explore processes in the same domain in relation to both migrant spouses and their non-migrant 'receiving' families, exposing interactions and enabling comparison.
The bract shaft with its hook extended was exposed for interaction with a testing substrate analogue, a loop of silk thread.
Disruption of the exposed PDZ interaction motif, either by further deletion of the carboxyl-terminal Leu-901 (GluA4ΔLP) or by mutation of Ser-899 and Leu-901 to alanine [GluA4 SL→AA)ΔP], abolished the interaction (Figure 2B).
The free thiol of Cys17 was used to immobilize C3d to the carboxymethylated surface of the SPR chip, ensuring an oriented, homogeneous immobilized C3d, with its ligand binding site exposed for interaction with CR2.
This is in contrast to the other known myosin E3 ligases, which target the head region of myosin [9], the portion of myosin exposed for interaction with actin and ATP.
Considering this vast area of growth, rice is constantly exposed to interaction with various organisms from insects to bacteria.
Our objective was to identify proteins that could be secreted to the extracellular compartment or exposed for interaction with host cell components.
The AP1 core adopts an activated, open conformation, with both of its cargo binding sites exposed for interaction with BST2CD-VpuCD.
